Journal: bioRxiv
Article Title: Structural basis of augment taurine uptake by taurine transporter alleviating cellular senescence
doi: 10.1101/2024.11.13.623519
Figure Lengend Snippet: a , Representative TauT and CK7 staining in bile duct tissues from normal liver. Scale bar, 100 μm. b , Representative staining of TauT in normal liver and cirrhosis liver. Scale bar, 50 μm. c , TauT protein expression levels in normal livers and cirrhosis livers. d , Immunohistochemistry evaluations of TauT expression in normal livers and cirrhosis livers. e , MFI quantifications of SA β-galactosidase expression in doxorubicin treated and transfected with TauT or TauT mutants of RBE cells. f , MFI quantifications of the proliferative cells in doxorubicin treated and transfected with TauT or TauT mutants of RBE cells. g-h , qPCR showing the relative expression of P16 ( g ) and P21 ( h ) in doxorubicin treated and transfected with wild type TauT or TauT-Δ464S of RBE cells. i-k, qPCR showing the relative expression of SASPs including CXCL1 ( i ), IL-6 ( j ), and IL-1β ( k ) in doxorubicin treated and transfected with wild type TauT or TauT-Δ464S of RBE cells. Data are mean ± s.e.m. from three independent assays, each with one measurement (n = 3). Statistical analysis was performed with Graph Pad Prism 10 using one-way ANOVA test. All values are means ± SEM. p ≤ 0.0001****,p ≤ 0.001***, p ≤ 0.01**, p ≤ 0.05*, p > 0.05ns are versus WT or control, all. Unlabeled groups represent no statistical difference. l , Volcano map of RBE cells transfected TauT ( SLC6A6 ) versus transfected vector. m , Western blot of TauT, ATDF3, P16 and P21 in doxorubicin treated and transfected with wild type TauT or TauT-Δ464S of RBE cells.
Article Snippet: Antibodies against the TauT (PA5-37460, Invitrogen, 1:1000 dilution), p16 (Abcam, Ab108349, 1:1000 dilution), p21 (Abcam, Ab109520, 1:1000 dilution), ATF3 (CST, mAb #18665, 1:1000 dilution), HRP-conjugated β-Actin (Aksomics, KC-5A08, 1:5000 dilution), and HRP-conjugated secondary antibodies (Aksomics, KC-RB-035, 1:5000 dilution) were used.
Techniques: Staining, Expressing, Immunohistochemistry, Transfection, Control, Plasmid Preparation, Western Blot
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